RiboRuler Low Range RNA Ladder
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RiboRuler Low Range RNA Ladder
Thermo Scientific™

RiboRuler Low Range RNA Ladder

Thermo Scientific RiboRuler Low Range RNA Ladder is a mixture of seven single-stranded RNA transcripts ranging from 100 to 1000深入閱讀
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產品號碼Quantity
SM18315 x 20 μL
產品號碼 SM1831
價格 (TWD)
-
申請報價
Quantity:
5 x 20 μL
Request bulk or custom format
Thermo Scientific RiboRuler Low Range RNA Ladder is a mixture of seven single-stranded RNA transcripts ranging from 100 to 1000 bp. They are chromatography-purified and produced from specific templates that contain a fragment of the pTZ19R polylinker and Lambda phage fragments, making them free from NTPs and RNA degradation products. The concentration of each RNA transcript is determined spectrophotometrically. To minimize the possibility of contamination during use, the ladder is aliquotted in smaller volumes.

Highlights:
• Sharp bands of uniform intensity
• Easy-to-remember band sizes and quantities (1000, 800, 600, 400, 300, 200and 100 bp)
• Approximate RNA quantification by predetermined concentration of ladder bands
• Supplied with 2X RNA Loading Dye

Applications:
• RNA sizing and quantification on native or denaturing gels
• Northern blotting
For Research Use Only. Not for use in diagnostic procedures.
規格
Concentration2X
Product LineRiboRuler
Product TypeDNA Ladder
Quantity5 x 20 μL
Gel TypeAgarose
Label or DyeXylene Cyanol FF, Bromophenol Blue, Ethidium Bromide
Size Range100 to 1000 bp
Unit Size5 x 20 µL
內容物與存放
• 5 x 20 μL RiboRuler Low Range RNA Ladder (in 1 mM EDTA, pH 6.0); sufficient for 50 uses
• 1 mL RNA Loading Dye

Store at -70°C or at -20°C (up to 6 months).

常見問答集 (常見問題)

What is the recommended voltage for electrophoresis with Thermo Scientific DNA and RNA ladders? What does V/cm mean?

Each of Thermo Scientific DNA and RNA ladders has a recommended voltage given in V/cm. Please refer to the gel picture in the product information sheet. V/cm stands for the amount of voltage recommended per cm of the distance between the two electrodes of the gel box (not gel length).

What are the end functional groups of Thermo Scientific DNA and RNA ladders?

Thermo Scientific DNA and RNA ladders have 5′-phosphate and 3′-hydroxyl groups.

Can I use Thermo Scientific 2X RNA Loading Dye for native gel electrophoresis?

Thermo Scientific 2X RNA Loading Dye contains 95% formamide as a denaturing agent and hence cannot be used for native gel electrophoresis.

What kind of RNA molecular weight ladders do you offer?

We offer the following RNA Ladders:


RiboRuler High Range RNA Ladder (Cat. No. SM1821)
RiboRuler Low Range RNA Ladder (Cat. No. SM1831)
Century Marker Templates, 100-500 bp (Cat. No. AM7780)
Century-Plus Marker Templates, 0.1-1 kb (Cat. No. AM7782)
Decade Markers, 10-150 bp (Cat. No. AM7778)
Millennium Markers-Formamide, 0.5-9 kb (Cat. No. AM7151)
RNA 6000 Ladder, 0.2-6 kb (Cat. No. AM7152)
RNA Century-Plus Markers, 0.1-1 kb (Cat. No. AM7145)
RNA Millennium Markers, 0.5-9 kb (Cat. No. AM7150)