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引用資料與參考文獻 (27)
Invitrogen™
FM™ 2-10 (N-(3-Triethylammoniumpropyl)-4-(4-(Diethylamino)styryl)Pyridinium Dibromide)
The FM™ 2-10 membrane probe is an excellent reagent both for identifying actively firing neurons and for investigating the mechanisms深入閱讀
| 產品號碼 | Quantity |
|---|---|
| T7508 | 5 mg |
產品號碼 T7508
價格 (TWD)
12,460.00
온라인 행사
Ends: 31-Dec-2025
17,800.00您節省 5,340.00 (30%)
5 mg
Quantity:
5 mg
價格 (TWD)
12,460.00
온라인 행사
Ends: 31-Dec-2025
17,800.00您節省 5,340.00 (30%)
5 mg
The FM™ 2-10 membrane probe is an excellent reagent both for identifying actively firing neurons and for investigating the mechanisms of activity-dependent vesicle cycling. This water-soluble dye, which is nontoxic to cells and virtually nonfluorescent in aqueous medium, is believed to insert into the outer leaflet of the cell membrane where it becomes intensely fluorescent. In a neuron that is actively releasing neurotransmitters, the dye becomes internalized within the recycled synaptic vesicles and the nerve terminals become brightly stained.
For Research Use Only. Not for use in diagnostic procedures.
規格
ColorOrange
Detection MethodFluorescence
For Use With (Equipment)Fluorescence Microscope
Product LineFM
Quantity5 mg
Shipping ConditionRoom Temperature
Label TypeFluorescent Dye
Product TypeMembrane Stain
SubCellular LocalizationPlasma Membrane
Unit Size5 mg
內容物與存放
Store at room temperature and protect from light.
常見問答集 (常見問題)
I want to study endosome trafficking using FM 4-64 dye. Will the label be retained after fixation? And can I label cells that have already been fixed?
引用資料與參考文獻 (27)
引用資料與參考文獻
Abstract
Ba2+ does not support synaptic vesicle retrieval in rat cerebrocortical synaptosomes.
Journal:Neurosci Lett
PubMed ID:9754790
To investigate whether any specific requirement for extracellular Ca2+ exists in the control synaptic vesicle retrieval, we examined the ability of the divalent cation Ba2+ to substitute for Ca2+ in both vesicle exocytosis and endocytosis. Ba2+ stimulated glutamate release from rat cerebrocortical synaptosomes. Ba2+-evoked release was inhibited by bafilomycin A1,
Frequency dependence of synaptic vesicle exocytosis in aortic baroreceptor neurons and the role of group III mGluRs.
Journal:Brain Res
PubMed ID:15051525
Synaptic transmission between baroreceptor afferents and the nucleus tractus solitarius (NTS) is essential for reflex regulation of blood pressure. High frequency stimulation of the afferents in vivo leads to a decrease in synaptic strength and is generally attributed to reduction in presynaptic neurotransmitter release. It has been hypothesized that during
Long-term staining of live Merkel cells with FM dyes.
Journal:Cell Tissue Res
PubMed ID:12658440
'Live Merkel cells in the skin and hair follicles are known to incorporate a fluorescence dye, quinacrine, which has been utilized to identify and dissect the cells for experiments. Quinacrine fluorescence of the cells is, however, quickly lost and quinacrine-stained Merkel cells soon become difficult to identify in tissue culture.
Two mechanisms of synaptic vesicle recycling in rat brain nerve terminals.
Journal:J Neurochem
PubMed ID:10987846
'KCl and 4-aminopyridine (4-AP) evoke glutamate release from rat brain cortical nerve terminals by voltage clamping or by Na(+) channel-generated repetitive action potentials, respectively. Stimulation by 4-AP but not KCl is largely mediated by protein kinase C (PKC). To determine whether KCl and 4-AP utilise the same mechanism to release
Calcium triggers calcineurin-dependent synaptic vesicle recycling in mammalian nerve terminals.
Journal:Curr Biol
PubMed ID:9651678
'BACKGROUND: Following exocytosis at the synapse, synaptic vesicle components are recovered by endocytosis. Morphological analysis has suggested that this occurs by a clathrin-mediated pathway, and the GTPase dynamin is thought to be involved in ''pinching off'' endocytosing vesicles. The finding that the calcium-dependent phosphatase calcineurin can dephosphorylate dynamin and two