TransFluoSpheres™ Carboxylate-Modified Microspheres, 0.04 μm (633/720), 2% solids
TransFluoSpheres™ Carboxylate-Modified Microspheres, 0.04 μm (633/720), 2% solids
Invitrogen™

TransFluoSpheres™ Carboxylate-Modified Microspheres, 0.04 μm (633/720), 2% solids

Microspheres (also called latex beads or latex particles) are spherical particles in the colloidal size range that are formed from深入閱讀
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產品號碼Quantity
T8870
亦稱為 T-8870
0.5 mL
產品號碼 T8870
亦稱為 T-8870
價格 (TWD)
15,470.00
Online offer
Ends: 31-Dec-2025
22,100.00
您節省 6,630.00 (30%)
0.5 mL
新增至購物車
Quantity:
0.5 mL
價格 (TWD)
15,470.00
Online offer
Ends: 31-Dec-2025
22,100.00
您節省 6,630.00 (30%)
0.5 mL
新增至購物車
Microspheres (also called latex beads or latex particles) are spherical particles in the colloidal size range that are formed from an amorphous polymer such as polystyrene. Our Molecular Probes™ TransFluoSpheres™ beads are manufactured using high-quality, ultraclean polystyrene and are loaded with two (or more) fluorescent dyes to produce a microspheres that can be excited by a commonly used excitation source (for example, the 633 nm spectral line of the Red He-Ne laser) but emit at much longer wavelengths, facilitating multicolor experiments and allowing the use of broadband excitation and emission filters. TransFluoSpheres™ microspheres typically show little or no photobleaching, even when excited with the intense illumination required for fluorescence microscopy.

TransFluoSpheres™ Microsphere Specifications:

• Label (Ex/Em): Red-fluorescent (633/720)
• Nominal bead diameter: 0.04 μm
• Coupling surface: Carboxylate
• Solids: 2%

Characteristics of the Carboxylate Coupling Surface
Carboxylate-modified FluoSpheres™ beads have a high density of pendent carboxylic acids on their surface, making them suitable for covalent coupling of proteins and other amine-containing biomolecules using water-soluble carbodiimide reagents such as EDAC.

Key Applications of Microspheres:
• Instrument calibration (flow cytometry, microscopy, HTS, HCS)
• Flow testing (microfluidics, blood flow, water flow, and air flow)
• Cell biology tracers (cell differentiation and cell tracing)
• Immunoassays (agglutination tests, ELISA, particle capture, and contrast reagents)

Choices for Fluorescent Microspheres
Among our complete offering of fluorescent microspheres products, you’ll find beads with these variations:
• Ten fluorescent colors
• Ten nominal bead diameters: 0.02 μm, 0.04 μm, 0.1 μm, 0.2 μm, 0.5 μm, 1.0 μm, 2.0 μm, 4.0 μm, 10.0 μm, and 15.0 μm
• Four surface modifications for protein coupling: carboxylate, sulfate, aldehyde-sulfate, amine
• Microspheres that are additionally precoupled with streptavidin, NeutrAvidin, biotin, europium, and platinum

Choices for Unstained Microspheres
We also offer hundreds of choices for UltraClean™ surfactant-free microspheres for research and commercial applications.

We’ll Make a Custom Microsphere Product for You
We will prepare custom orders upon request. Our custom conjugation service is efficient and confidential, and we stand by the quality of our work. We are ISO 13485:2000 certified.

For Research Use Only. Not intended for animal or human therapeutic or diagnostic use.
For Research Use Only. Not for use in diagnostic procedures.
規格
Product LineTransFluoSpheres
Quantity0.5 mL
Shipping ConditionRoom Temperature
Surface ModificationCarboxylate
ColorFar-red
Diameter (Metric)0.04 μm
MaterialPolystyrene
Product TypeCarboxylate-Modified Microsphere
Unit Size0.5 mL
內容物與存放
Store in refrigerator (2–8°C) and protect from light.

常見問答集 (常見問題)

What is the warranty for FluoSpheres microspheres?

The warranty period for FluoSpheres microspheres is 1-year from the date of shipment.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

After washing and centrifugation, there was only a very small pellet left of my microsphere beads and the solution was transparent. Why is this?

Centrifugation is not an effective way to collect smaller microspheres; many particles remain in the solution even if you can visualize a small pellet. For beads less than 1 µm in diameter, we recommend washing by either:

Cross-flow filtration, as these particles have a very high compression modulus and can withstand high g-forces without risk of harm or dialysis with a 500 kDa MWCO
Note: Microspheres greater than 1 µm in diameter can be centrifuged at 1,300 rpm.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I've had my microspheres for over a year, and I'm wondering if they're still good to use. What are some good ways to check their functionality?

Bacterial contamination is the most common cause of microspheres becoming unusable. Many of our particles are supplied with a low level of sodium azide to prevent bacterial contamination, but sometimes this can still occur. Bacterial contamination is best assessed by plating on appropriate growth medium and checking the plates after 72 hr.

Find additional tips, troubleshooting help, and resources within ourMicrospheres Support Center.

I accidentally froze my microspheres; can I still use them?

Even brief freezing can cause irreversible aggregation and potential distortion of the bead shape. You should not use these microspheres.

Find additional tips, troubleshooting help, and resources within our Microspheres Support Center.

My protein-coated microspheres appear to be non-specifically binding in my experimental system. Do you have a product that can help reduce these non-specific interactions?

Non-specific binding can often be relieved by a blocking solution, but microspheres seem to require a stronger blocking solution than those most commonly commercially available. Hence, we've developed the BlockAid Blocking Solution (Cat. No. B10710). This reagent is a protein-based blocking solution designed for use with FluoSpheres microspheres and TransFluoSpheres microspheres conjugated to biotin, streptavidin, NeutrAvidin biotin-binding protein, or other proteins. The BlockAid Blocking Solution has proven useful for reducing the nonspecific binding of protein-coated or other macromolecule-coated microspheres in a wide variety of flow cytometry, microscopy, and microarray applications.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

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