The amplified product spans an exon junction and the probe and one of the primers sit within one exon. This assay may detect gDNA.
| Interrogated Sequence | Translated Protein | Exon Boundary | Assay Location | Amplicon Length | |
|---|---|---|---|---|---|
| RefSeq | XM_003434858.3 | XP_003434906.1 | 3 - 3 | 419 | 133 |
| XM_022420081.1 | XP_022275789.1 | 3 - 3 | 376 | 133 | |