The amplified product spans an exon junction and the probe and one of the primers sit within one exon. This assay may detect gDNA.
Interrogated Sequence | Translated Protein | Exon Boundary | Assay Location | Amplicon Length | |
---|---|---|---|---|---|
RefSeq | NM_001242.4 | NP_001233.1 | 3 - 3 | 494 | 70 |
XM_017020232.1 | XP_016875721.1 | 3 - 3 | 512 | 70 | |
XM_017020234.1 | XP_016875723.1 | 3 - 3 | 512 | 70 | |
GenBank mRNA | AK315732.1 | - | 3 - 3 | 513 | 70 |
BC012160.1 | - | 3 - 3 | 480 | 70 | |
M63928.1 | - | 3 - 3 | 383 | 70 |