The amplified product spans an exon junction and the probe and one of the primers sit within one exon. This assay may detect gDNA.
| Interrogated Sequence | Translated Protein | Exon Boundary | Assay Location | Amplicon Length | |
|---|---|---|---|---|---|
| RefSeq | NM_001185172.1 | NP_001172101.1 | 2 - 3 | 121 | 83 |
| XM_021088627.1 | XP_020944286.1 | - | 168 | 83 | |
| GenBank mRNA | AK344783.1 | - | 2 - 3 | 97 | 83 |
| AY610483.1 | - | 1 - 2 | 97 | 83 | |
| EV895402.2 | - | 1 - 2 | 48 | 83 | |
| EV897222.2 | - | 2 - 3 | 130 | 83 | |