Assay Design

The assay probe spans an exon junction. This assay will detect gDNA.

Gene Transcripts

Gene Symbol: AT-IE
Entrez Gene ID: 840076
Gene Name: histidine biosynthesis bifunctional protein (HISIE)
Gene Aliases: AT1G31860, F5M6.13, F5M6_13, HISN2, HISTIDINE BIOSYNTHESIS 2, PHOSPHORIBOSYL-ATP PYROPHOSPHOHYDROLASE
Chromosome Location: Chr.1: 11434060 - 11436607 on Build TAIR10.1

Interrogated Sequence		Translated Protein	Exon Boundary	Assay Location	Amplicon Length
RefSeq	NM_001332983.1	NP_001322576.1	2 - 3	1290	154
	NM_001332984.1	NP_001322577.1	2 - 3	471	154
	NM_102923.4	NP_174469.1	2 - 3	471	154
GenBank mRNA	AB006082.1	-	2 - 3	439	154
	AY046000.1	-	2 - 3	465	154
	AY079376.1	-	2 - 3	394	154
	AY086514.1	-	2 - 3	471	154