Assay Design

The assay probe spans an exon junction. This assay will detect gDNA.

Gene Transcripts

Gene Symbol: NIT1
Entrez Gene ID: 823556
Gene Name: nitrilase 1
Gene Aliases: A. THALIANA NITRILASE 1, AT3G44310, ATNIT1, NITI, NITRILE AMINOHYDROLASE, nitrilase 1
Chromosome Location: Chr.3: 15986624 - 15989505 on Build TAIR10.1

Interrogated Sequence		Translated Protein	Exon Boundary	Assay Location	Amplicon Length
RefSeq	NM_001084765.1	NP_001078234.1	4 - 5	916	170
	NM_001339127.1	NP_001319680.1	3 - 4	1047	170
	NM_001339128.1	NP_001325848.1	3 - 4	1009	170
	NM_180680.3	NP_851011.1	4 - 5	1156	170
GenBank mRNA	AB493638.1	-	4 - 5	879	170
	AY042847.1	-	4 - 5	914	170
	AY084633.1	-	3 - 4	798	170
	BT000040.1	-	4 - 5	879	170
	DQ446730.1	-	4 - 5	879	170