The amplified product spans an exon junction and the probe and one of the primers sit within one exon. This assay may detect gDNA.
| Interrogated Sequence | Translated Protein | Exon Boundary | Assay Location | Amplicon Length | |
|---|---|---|---|---|---|
| RefSeq | XM_022427724.1 | XP_022283432.1 | 3 - 3 | 213 | 135 |
| XM_532539.4 | XP_532539.1 | 3 - 3 | 668 | 135 | |