The amplified product spans an exon junction and the probe and one of the primers sit within one exon. This assay may detect gDNA.
| Interrogated Sequence | Translated Protein | Exon Boundary | Assay Location | Amplicon Length | |
|---|---|---|---|---|---|
| RefSeq | XM_022415274.1 | XP_022270982.1 | 2 - 2 | 598 | 135 |
| XM_022415275.1 | XP_022270983.1 | 3 - 3 | 386 | 135 | |
| XM_022415276.1 | XP_022270984.1 | 3 - 3 | 527 | 135 | |
| XM_022415277.1 | XP_022270985.1 | 2 - 2 | 598 | 135 | |
| XM_854989.5 | XP_860082.1 | 2 - 2 | 598 | 135 | |