Assay Design

The assay probe spans an exon junction. This assay will detect gDNA.

Gene Transcripts

Gene Symbol: Sh
Entrez Gene ID: 32780
Gene Name: Shaker
Gene Aliases: BcDNA:GH03046, CG12348, CG17860, CG7640, Dmel\CG12348, Dmel_CG12348, EKO, Eko, F-c, K[[v]]1, Kv1, SH, Sha, Shw, V-I, minisleep, sh, sha
Chromosome Location: Chr.X: 17924307 - 18063247 on Build Release_6_plus_ISO1_MT
Species Specific ID (Flybase ID): FBgn0003380

Interrogated Sequence		Translated Protein	Exon Boundary	Assay Location	Amplicon Length
RefSeq	NM_001169317.1	NP_001162788.1	2 - 3	371	101
	NM_001201739.2	NP_001188668.1	4 - 5	2663	101
	NM_001258797.1	NP_001245726.1	3 - 4	1306	101
	NM_001258798.2	NP_001245727.1	6 - 7	2270	101
	NM_001272735.3	NP_001259664.1	4 - 5	2663	101
	NM_001272736.2	NP_001259665.2	3 - 4	851	101
	NM_001272737.1	NP_001259666.2	3 - 4	1626	101
	NM_001316640.1	NP_001303569.1	3 - 4	916	101
	NM_078669.5	NP_523393.3	3 - 4	916	101
	NM_167592.4	NP_728120.1	3 - 4	450	101
	NM_167594.4	NP_728122.1	4 - 5	2663	101
	NM_167595.4	NP_728123.1	3 - 4	916	101
	NM_167596.5	NP_728124.1	3 - 4	450	101
	NM_206774.2	NP_996497.1	3 - 4	859	101
	NM_206775.5	NP_996498.2	6 - 7	2270	101
GenBank mRNA	BT050432.1	-	3 - 4	450	101
	X06184.1	-	-	369	101
	X07132.1	-	-	544	101
	X07133.1	-	-	248	101
	X07134.1	-	-	859	101