Assay Design

The assay probe spans an exon junction. This assay will detect gDNA.

Gene Transcripts

Gene Symbol: blot
Entrez Gene ID: 39939
Gene Name: bloated tubules
Gene Aliases: CG3897, DmBLOT, Dmel\CG3897, Dmel_CG3897, l(3)1658
Chromosome Location: Chr.3L: 17396031 - 17421514 on Build Release_6_plus_ISO1_MT
Species Specific ID (Flybase ID): FBgn0027660

Interrogated Sequence		Translated Protein	Exon Boundary	Assay Location	Amplicon Length
RefSeq	NM_001300164.1	NP_001287093.1	5 - 6	794	65
	NM_079401.3	NP_524125.2	5 - 6	1759	65
	NM_170636.2	NP_730263.1	5 - 6	1329	65
	NM_170637.2	NP_730264.1	4 - 5	632	65
	NM_170638.2	NP_730265.1	4 - 5	762	65
GenBank mRNA	BT033062.1	-	4 - 5	632	65
	BT133104.1	-	5 - 6	797	65
	BT133440.1	-	5 - 6	1709	65