Assay Design

The assay probe spans an exon junction. This assay will detect gDNA.

Gene Transcripts

Gene Symbol: Hcs
Entrez Gene ID: 40659
Gene Name: Holocarboxylase synthetase
Gene Aliases: CG14670, Dmel\CG14670, Dmel_CG14670, HCS, HSC, NEST:bs27h05
Chromosome Location: Chr.3R: 5474899 - 5485393 on Build Release_6_plus_ISO1_MT
Species Specific ID (Flybase ID): FBgn0037332

Interrogated Sequence		Translated Protein	Exon Boundary	Assay Location	Amplicon Length
RefSeq	NM_001275355.1	NP_001262284.1	7 - 8	2358	68
	NM_001300246.1	NP_001287175.1	7 - 8	2520	68
	NM_001300247.1	NP_001287176.1	7 - 8	2376	68
	NM_141285.2	NP_649542.1	7 - 8	2502	68
GenBank mRNA	AY118365.1	-	7 - 8	2499	68