Assay Design

The assay primers and probes lie within a single exon. This assay will detect gDNA.

Gene Transcripts

Gene Symbol: kermit
Entrez Gene ID: 46027
Gene Name: CG11546-PA
Gene Aliases: BEST:LD04728, CG11546, Dmel\CG11546, Dmel_CG11546, LD04728, dGIPC, dKermit, l(2)02045
Chromosome Location: Chr.2R: 8146918 - 8156654 on Build Release_6_plus_ISO1_MT
Species Specific ID (Flybase ID): FBgn0010504

Interrogated Sequence		Translated Protein	Exon Boundary	Assay Location	Amplicon Length
RefSeq	NM_001299258.1	NP_001286187.1	1 - 1	1439	146
	NM_143771.4	NP_652028.1	2 - 2	1856	146
	NM_165590.3	NP_724649.1	2 - 2	1497	146
	NM_165591.3	NP_724650.1	2 - 2	1500	146
	NM_165592.3	NP_724651.1	2 - 2	1384	146
GenBank mRNA	AY060451.1	-	2 - 2	1777	146