The amplified product spans an exon junction and the probe and one of the primers sit within one exon. This assay may detect gDNA.
| Interrogated Sequence | Translated Protein | Exon Boundary | Assay Location | Amplicon Length | |
|---|---|---|---|---|---|
| RefSeq | NM_001031357.1 | NP_001026528.1 | 3 - 4 | 432 | 79 |
| XM_015290581.2 | XP_015146067.1 | 3 - 4 | 489 | 79 | |
| XM_015290585.2 | XP_015146071.1 | 3 - 4 | 416 | 79 | |
| GenBank mRNA | AJ720525.1 | - | 3 - 4 | 432 | 79 |