The assay primers and probes lie within a single exon. This assay will detect gDNA.
| Interrogated Sequence | Translated Protein | Exon Boundary | Assay Location | Amplicon Length | |
|---|---|---|---|---|---|
| RefSeq | NM_000663.4 | NP_000654.2 | 16 - 16 | 4660 | 95 |
| NM_001127448.1 | NP_001120920.1 | 16 - 16 | 3982 | 95 | |
| NM_020686.5 | NP_065737.2 | 16 - 16 | 3888 | 95 | |
| XM_011522400.2 | XP_011520702.1 | 17 - 17 | 4228 | 95 | |
| XM_011522401.2 | XP_011520703.1 | 17 - 17 | 4189 | 95 | |
| GenBank mRNA | AF237813.1 | - | 1 - 1 | 1578 | 95 |
| AL137454.1 | - | 1 - 1 | 44 | 95 | |
| BC008990.1 | - | 1 - 1 | 338 | 95 | |
| BX537362.1 | - | 16 - 16 | 3885 | 95 | |