The amplified product spans an exon junction and the probe and one of the primers sit within one exon. This assay may detect gDNA.
| Interrogated Sequence | Translated Protein | Exon Boundary | Assay Location | Amplicon Length | |
|---|---|---|---|---|---|
| RefSeq | NM_001033557.2 | NP_001028729.1 | 4 - 4 | 1523 | 84 |
| NM_002706.5 | NP_002697.1 | 4 - 4 | 1523 | 84 | |
| NM_177968.3 | NP_808907.1 | 4 - 4 | 1523 | 84 | |
| NM_177969.3 | NP_808908.1 | 3 - 3 | 663 | 84 | |
| XM_011532936.2 | XP_011531238.1 | 4 - 4 | 1481 | 84 | |
| XM_017004395.1 | XP_016859884.1 | 4 - 4 | 1547 | 84 | |
| XM_017004396.1 | XP_016859885.1 | 4 - 4 | 1547 | 84 | |
| XM_017004397.1 | XP_016859886.1 | 4 - 4 | 1547 | 84 | |
| GenBank mRNA | AF294792.1 | - | 3 - 3 | 1037 | 84 |
| AJ005801.1 | - | 3 - 3 | 1037 | 84 | |
| AJ271832.1 | - | 4 - 4 | 1430 | 84 | |
| AJ271835.1 | - | 4 - 4 | 1430 | 84 | |
| AK056009.1 | - | 4 - 4 | 1459 | 84 | |
| AK291214.1 | - | 4 - 4 | 1465 | 84 | |
| AL833035.1 | - | 4 - 4 | 1328 | 84 | |
| BC012002.1 | - | 3 - 3 | 565 | 84 | |
| BC064381.1 | - | 4 - 4 | 1438 | 84 | |
| DQ023508.1 | - | 4 - 4 | 1449 | 84 | |
| DQ023510.1 | - | 4 - 4 | 1449 | 84 | |