Assay Design

The assay probe spans an exon junction. This assay will detect gDNA.

Gene Transcripts

Gene Symbol
PFKM
Entrez Gene ID
5213
Gene Name
phosphofructokinase, muscle
Gene Aliases
ATP-PFK, GSD7, PFK-1, PFK1, PFKA, PFKX, PPP1R122
Chromosome Location
Chr.12: 48105278 - 48146404 on Build GRCh38
Interrogated Sequence Translated Protein Exon Boundary Assay Location Amplicon Length
RefSeq NM_000289.5 NP_000280.1 14 - 15 1530 106
NM_001166686.1 NP_001160158.1 16 - 17 1832 106
NM_001166687.1 NP_001160159.1 14 - 15 1384 106
NM_001166688.1 NP_001160160.1 13 - 14 1473 106
XM_005268974.1 XP_005269031.1 17 - 18 1729 106
XM_005268975.1 XP_005269032.1 17 - 18 1868 106
XM_005268976.3 XP_005269033.1 17 - 18 1911 106
XM_005268977.1 XP_005269034.1 16 - 17 1715 106
XM_005268978.2 XP_005269035.1 16 - 17 1628 106
XM_005268979.1 XP_005269036.1 16 - 17 1715 106
XM_011538487.1 XP_011536789.1 15 - 16 1570 106
XM_011538488.2 XP_011536790.1 14 - 15 1495 106
XM_017019467.1 XP_016874956.1 17 - 18 1798 106
XM_017019468.1 XP_016874957.1 16 - 17 1650 106
XM_017019469.1 XP_016874958.1 15 - 16 1622 106
GenBank mRNA AK126020.1 - 18 - 19 2185 106
AK126229.1 - 17 - 18 1782 106
AK303681.1 - 9 - 10 877 106
AK308185.1 - 9 - 10 853 106
AK312284.1 - 14 - 15 1412 106
BC000534.2 - 13 - 14 1360 106
BC007798.2 - 12 - 13 1247 106
BC012799.2 - 13 - 14 1360 106
BC013298.2 - 13 - 14 1360 106
BC021203.2 - 14 - 15 1382 106
CR541698.1 - 4 - 5 400 106
J05533.1 - 5 - 6 441 106
KU178251.1 - 13 - 14 1345 106
KU178252.1 - 12 - 13 1252 106
KU178253.1 - 13 - 14 1345 106
KU178254.1 - 2 - 3 190 106
M26066.1 - 14 - 15 1421 106
U24183.1 - 13 - 14 1673 106
Y00698.1 - 13 - 14 1364 106

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