Assay Design

The assay primers and probes lie within a single exon. This assay will detect gDNA.

Gene Transcripts

Gene Symbol
MIS12
Entrez Gene ID
79003
Gene Name
MIS12, kinetochore complex component
Gene Aliases
2510025F08Rik, KNTC2AP, MTW1, hMis12
Chromosome Location
Chr.17: 5486374 - 5490814 on Build GRCh38
Interrogated Sequence Translated Protein Exon Boundary Assay Location Amplicon Length
RefSeq NM_001258217.1 NP_001245146.1 3 - 3 2533 145
NM_001258218.1 NP_001245147.1 2 - 2 2139 145
NM_001258219.1 NP_001245148.1 3 - 3 2269 145
NM_001258220.1 NP_001245149.1 2 - 2 1875 145
NM_024039.2 NP_076944.1 3 - 3 2375 145
XM_005256797.2 XP_005256854.1 3 - 3 2697 145
XM_017025033.1 XP_016880522.1 2 - 2 3553 145
XM_017025034.1 XP_016880523.1 3 - 3 3052 145
XM_017025035.1 XP_016880524.1 4 - 4 2543 145
GenBank mRNA AL136906.1 - 3 - 3 2341 145
BC000229.1 - 2 - 2 1929 145

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