The amplified product spans an exon junction and the probe and one of the primers sit within one exon. This assay may detect gDNA.
| Interrogated Sequence | Translated Protein | Exon Boundary | Assay Location | Amplicon Length | |
|---|---|---|---|---|---|
| RefSeq | NM_002818.2 | NP_002809.2 | 3 - 3 | 168 | 95 |
| XM_006720213.1 | XP_006720276.1 | 3 - 3 | 962 | 219 | |
| GenBank mRNA | AK026580.1 | - | 3 - 3 | 189 | 95 |
| BC004368.1 | - | 3 - 3 | 118 | 95 | |
| BC019885.1 | - | 3 - 3 | 118 | 95 | |
| BX161498.1 | - | 3 - 3 | 743 | 95 | |
| CR541657.1 | - | 3 - 3 | 103 | 95 | |
| CR541743.1 | - | 3 - 3 | 103 | 95 | |