The amplified product spans an exon junction and the probe and one of the primers sit within one exon. This assay may detect gDNA.
| Interrogated Sequence | Translated Protein | Exon Boundary | Assay Location | Amplicon Length | |
|---|---|---|---|---|---|
| RefSeq | NM_181503.2 | NP_852480.1 | 9 - 9 | 766 | 168 |
| XM_006719763.1 | XP_006719826.1 | 8 - 8 | 897 | 168 | |
| GenBank mRNA | AF025438.1 | - | 8 - 8 | 495 | 168 |
| AK096810.1 | - | 7 - 7 | 2406 | 168 | |
| AL050353.1 | - | 8 - 8 | 1989 | 168 | |
| BC020773.1 | - | 9 - 9 | 527 | 168 | |