Assay Design

The assay primers and probes lie within a single exon. This assay will detect gDNA.

Gene Transcripts

Interrogated Sequence		Translated Protein	Exon Boundary	Assay Location	Amplicon Length
RefSeq	NM_000053.3	NP_000044.2	21 - 21	6362	100
	NM_001005918.2	NP_001005918.1	17 - 17	5741	100
	NM_001243182.1	NP_001230111.1	22 - 22	6029	100
	XM_005266423.2	XP_005266480.1	21 - 21	6267	100
	XM_005266424.4	XP_005266481.1	21 - 21	6888	100
	XM_005266427.2	XP_005266484.1	20 - 20	6190	100
	XM_005266428.1	XP_005266485.1	19 - 19	6172	100
	XM_005266430.4	XP_005266487.1	22 - 22	6370	100
	XM_005266431.3	XP_005266488.1	21 - 21	6422	100
	XM_005266432.2	XP_005266489.1	18 - 18	5938	100
	XM_006719837.3	XP_006719900.1	21 - 21	6516	100
	XM_006719838.1	XP_006719901.1	15 - 15	4157	100
	XM_006719839.1	XP_006719902.1	14 - 14	3974	100
	XM_011535117.2	XP_011533419.1	21 - 21	7443	100
	XM_011535118.1	XP_011533420.1	20 - 20	6289	100
	XM_011535119.1	XP_011533421.1	20 - 20	6241	100
	XM_011535122.2	XP_011533424.1	20 - 20	5115	100
	XM_017020627.1	XP_016876116.1	21 - 21	6266	100
	XM_017020628.1	XP_016876117.1	20 - 20	5111	100
GenBank mRNA	BX648261.1	-	14 - 14	5553	100
	L25591.1	-	16 - 16	5138	100
	U11700.1	-	21 - 21	6366	100