Assay Design

The assay primers and probes lie within a single exon. This assay will detect gDNA.

Gene Transcripts

Gene Symbol: POLR2F
Entrez Gene ID: 5435
Gene Name: RNA polymerase II subunit F
Gene Aliases: HRBP14.4, POLRF, RPABC14.4, RPABC2, RPB14.4, RPB6, RPC15
Chromosome Location: Chr.22: 37953663 - 38041915 on Build GRCh38

Interrogated Sequence		Translated Protein	Exon Boundary	Assay Location	Amplicon Length
RefSeq	NM_001301130.1	NP_001288059.1	6 - 6	867	68
	NM_001301131.1	NP_001288060.1	5 - 5	708	68
	XM_017028832.1	XP_016884321.1	6 - 6	755	68
GenBank mRNA	AK026327.1	-	1 - 1	1054	68

Non Gene Transcripts

Interrogated Sequence		Translated Protein	Exon Boundary	Assay Location	IMAGE Clone ID	Transcript Location	Amplicon Length
GenBankmRNA	AW297329.1	-	1	167		Chr. 22 - 38041191 - 38041595 [+] on Build GRCh38	68

Adjacent Gene Details

	Gene Name	Gene Symbol	Alias	Distance	Relative Direction	Location Chromosome
Upstream	microRNA 4534	MIR4534		-51.5kb	Sense	Chr. - 37988794 - 37988853 on Build GRCh38
Downstream	protein interacting with PRKCA 1	PICK1	PICK, PRKCABP	14.5kb	Sense	Chr. - 38056311 - 38075704 on Build GRCh38

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