Assay Design

The assay primers and probes lie within a single exon. This assay will detect gDNA.

Gene Transcripts

Gene Symbol: LEF1
Entrez Gene ID: 51176
Gene Name: lymphoid enhancer binding factor 1
Gene Aliases: LEF-1, TCF10, TCF1ALPHA, TCF7L3
Chromosome Location: Chr.4: 108047545 - 108168956 on Build GRCh38

Interrogated Sequence		Translated Protein	Exon Boundary	Assay Location	Amplicon Length
GenBank mRNA	AK128255.1	-	2 - 2	1655	98

Non Gene Transcripts

Interrogated Sequence		Translated Protein	Exon Boundary	Assay Location	IMAGE Clone ID	Transcript Location	Amplicon Length
GenBankmRNA	DA383766.1	-	1	396		Chr. 4 - 108048830 - 108049463 [-] on Build GRCh38	98

Adjacent Gene Details

	Gene Name	Gene Symbol	Alias	Distance	Relative Direction	Location Chromosome
Upstream	hydroxyacyl-CoA dehydrogenase	HADH	HAD, HADH1, HADHSC, HCDH, HHF4, MSCHAD, SCHAD	-12.4kb	Anti-sense	Chr. - 107989714 - 108035175 on Build GRCh38
Downstream	LEF1 antisense RNA 1	LEF1-AS1	LEF1NAT	117.3kb	Anti-sense	Chr. - 108167525 - 108176430 on Build GRCh38

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