The amplified product spans an exon junction and the probe and one of the primers sit within one exon. This assay may detect gDNA.
| Interrogated Sequence | Translated Protein | Exon Boundary | Assay Location | Amplicon Length | |
|---|---|---|---|---|---|
| RefSeq | NM_001293212.1 | NP_001280141.1 | 3 - 3 | 618 | 117 |
| NM_001293213.1 | NP_001280142.1 | 3 - 3 | 534 | 117 | |
| NM_001293214.1 | NP_001280143.1 | 2 - 2 | 402 | 117 | |
| NM_001293215.1 | NP_001280144.1 | 3 - 3 | 521 | 117 | |
| NM_001293216.1 | NP_001280145.1 | 3 - 3 | 420 | 117 | |
| NM_178014.3 | NP_821133.1 | 3 - 3 | 534 | 117 | |
| GenBank mRNA | AB062393.1 | - | 3 - 3 | 360 | 117 |
| AF070561.1 | - | 3 - 3 | 355 | 117 | |
| AF070593.1 | - | 3 - 3 | 355 | 117 | |
| AF070600.1 | - | 3 - 3 | 372 | 117 | |
| AF141349.1 | - | 3 - 3 | 372 | 117 | |
| AK098772.1 | - | 3 - 3 | 379 | 117 | |
| AK297489.1 | - | 3 - 3 | 288 | 117 | |
| AK298889.1 | - | 3 - 3 | 424 | 117 | |
| AK302314.1 | - | 3 - 3 | 487 | 117 | |
| AK316265.1 | - | 3 - 3 | 291 | 117 | |
| AK316316.1 | - | 3 - 3 | 416 | 117 | |
| BC001938.1 | - | 3 - 3 | 359 | 117 | |
| BC002347.2 | - | 3 - 3 | 364 | 117 | |
| BC005838.2 | - | 3 - 3 | 359 | 117 | |
| BC007605.1 | - | 3 - 3 | 364 | 117 | |
| BC013374.2 | - | 3 - 3 | 355 | 117 | |
| BC019924.1 | - | 3 - 3 | 352 | 117 | |
| BC020946.1 | - | 3 - 3 | 393 | 117 | |
| BC021909.1 | - | 3 - 3 | 354 | 117 | |
| BC070326.1 | - | 3 - 3 | 370 | 117 | |
| BC103746.1 | - | 3 - 3 | 377 | 117 | |
| BU557299.1 | - | 3 - 3 | 534 | 117 | |
| DC353572.1 | - | 3 - 3 | 522 | 117 | |
| DC389555.1 | - | 2 - 2 | 246 | 117 | |
| DC405540.1 | - | 3 - 3 | 331 | 117 | |
| V00599.1 | - | 2 - 2 | 207 | 117 | |