Assay Design

The assay primers and probes lie within a single exon. This assay will detect gDNA.

Gene Transcripts

Gene Symbol: HSD3B1
Entrez Gene ID: 3283
Gene Name: hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 1
Gene Aliases: 3BETAHSD, HSD3B, HSDB3, HSDB3A, I, SDR11E1
Chromosome Location: Chr.1: 119507112 - 119515060 on Build GRCh38

Interrogated Sequence		Translated Protein	Exon Boundary	Assay Location	Amplicon Length
RefSeq	NM_000862.2	NP_000853.1	4 - 4	752	112
	XM_011541314.2	XP_011539616.1	4 - 4	793	112
	XM_017001119.1	XP_016856608.1	4 - 4	716	112
GenBank mRNA	AK291556.1	-	3 - 3	766	112
	AL544564.3	-	4 - 4	752	112
	AL550307.3	-	4 - 4	734	112
	BC031999.1	-	3 - 3	694	112
	M27137.1	-	3 - 3	610	112
	M35493.1	-	3 - 3	637	112
	S45679.1	-	4 - 4	738	112
	X53321.1	-	3 - 3	637	112
	X55997.1	-	3 - 3	607	112