The amplified product spans an exon junction and the probe and one of the primers sit within one exon. This assay may detect gDNA.
Interrogated Sequence | Translated Protein | Exon Boundary | Assay Location | Amplicon Length | |
---|---|---|---|---|---|
RefSeq | NM_001142448.1 | NP_001135920.1 | 2 - 2 | 151 | 96 |
XM_006721096.3 | XP_006721159.1 | 2 - 2 | 158 | 96 | |
XM_017023757.1 | XP_016879246.1 | 2 - 2 | 159 | 96 | |
XM_017023758.1 | XP_016879247.1 | 2 - 2 | 155 | 96 | |
XM_017023759.1 | XP_016879248.1 | 2 - 2 | 154 | 96 | |
GenBank mRNA | BC038961.1 | - | 2 - 2 | 165 | 96 |
BC065235.1 | - | 2 - 2 | 133 | 96 |