The amplified product spans an exon junction and the probe and one of the primers sit within one exon. This assay may detect gDNA.
| Interrogated Sequence | Translated Protein | Exon Boundary | Assay Location | Amplicon Length | |
|---|---|---|---|---|---|
| RefSeq | NM_001289746.1 | NP_001276675.1 | 2 - 2 | 229 | 122 |
| NM_002046.5 | NP_002037.2 | 3 - 3 | 243 | 122 | |
| GenBank mRNA | AB062273.1 | - | 3 - 3 | 107 | 122 |
| AF261085.1 | - | 3 - 3 | 158 | 122 | |
| AK026525.1 | - | 3 - 3 | 180 | 122 | |
| AK299448.1 | - | 3 - 3 | 128 | 122 | |
| AK308198.1 | - | 3 - 3 | 131 | 122 | |
| AY007133.1 | - | 3 - 3 | 107 | 122 | |
| BC001601.1 | - | 3 - 3 | 109 | 122 | |
| BC004109.2 | - | 3 - 3 | 107 | 122 | |
| BC009081.1 | - | 3 - 3 | 116 | 122 | |
| BC013310.2 | - | 3 - 3 | 115 | 122 | |
| BC020308.1 | - | 2 - 2 | 93 | 122 | |
| BC025925.1 | - | 3 - 3 | 112 | 122 | |
| BC026907.1 | - | 2 - 2 | 93 | 122 | |
| BC029340.1 | - | 3 - 3 | 101 | 122 | |
| BC029618.1 | - | 3 - 3 | 109 | 122 | |
| BC083511.1 | - | 3 - 3 | 107 | 122 | |
| BU155402.1 | - | 3 - 3 | 280 | 122 | |
| EU668321.1 | - | 3 - 3 | 157 | 122 | |
| HY022295.1 | - | 2 - 2 | 206 | 122 | |
| M17851.1 | - | 2 - 2 | 88 | 124 | |
| M33197.1 | - | 3 - 3 | 115 | 122 | |
| X53778.1 | - | 3 - 3 | 111 | 122 | |