The amplified product spans an exon junction and the probe and one of the primers sit within one exon. This assay may detect gDNA.
| Interrogated Sequence | Translated Protein | Exon Boundary | Assay Location | Amplicon Length | |
|---|---|---|---|---|---|
| GenBank mRNA | AK298272.1 | - | 7 - 7 | 904 | 127 |
| AK304648.1 | - | 5 - 5 | 807 | 127 | |
| BC109053.1 | - | 6 - 6 | 933 | 127 | |
| BT019657.1 | - | 5 - 5 | 734 | 127 | |
| M34960.1 | - | 5 - 5 | 743 | 127 | |
| M55654.1 | - | 6 - 6 | 984 | 127 | |
| X54993.1 | - | 5 - 5 | 829 | 127 | |