Assay Design

The assay primers and probes lie within a single exon. This assay will detect gDNA.

Gene Transcripts

Gene Symbol
Fem1a
Entrez Gene ID
14154
Gene Name
feminization 1 homolog a (C. elegans)
Gene Aliases
AW611390, Fem1aa
Chromosome Location
Chr.17: 56256793 - 56263608 on Build GRCm38
Interrogated Sequence Translated Protein Exon Boundary Assay Location Amplicon Length
RefSeq NM_010192.4 NP_034322.3 1 - 1 568 58
GenBank mRNA AK005041.1 - 1 - 1 527 58
AK034412.1 - 1 - 1 543 58
AK080978.1 - 1 - 1 540 58
AK129000.1 - 1 - 1 551 58
AK140346.1 - - 58
AK147879.1 - 1 - 1 553 58
AK155363.1 - 1 - 1 552 58
BC009161.1 - 1 - 1 535 58
BC054382.1 - 1 - 1 539 58
BC110669.1 - 1 - 1 522 58

Gene Symbol
4931440F15Rik
Entrez Gene ID
216622
Gene Name
RIKEN cDNA 4931440F15 gene
Gene Aliases
Fem1ab
Chromosome Location
Chr.11: 29822395 - 29825668 on Build GRCm38
Interrogated Sequence Translated Protein Exon Boundary Assay Location Amplicon Length
RefSeq NM_176829.2 NP_789799.1 1 - 1 665 58
GenBank mRNA AK029918.1 - 1 - 1 665 58
BC139107.1 - 1 - 1 522 58
BC139123.1 - 1 - 1 522 58

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