The amplified product spans an exon junction and the probe and one of the primers sit within one exon. This assay may detect gDNA.
| Interrogated Sequence | Translated Protein | Exon Boundary | Assay Location | Amplicon Length | |
|---|---|---|---|---|---|
| RefSeq | NM_016879.2 | NP_058575.2 | 6 - 6 | 1050 | 86 |
| GenBank mRNA | AF021836.1 | - | 6 - 6 | 1034 | 86 |
| AK009099.1 | - | 6 - 6 | 1049 | 86 | |
| AK028825.1 | - | 6 - 6 | 1050 | 86 | |