The amplified product spans an exon junction and the probe and one of the primers sit within one exon. This assay may detect gDNA.
| Interrogated Sequence | Translated Protein | Exon Boundary | Assay Location | Amplicon Length | |
|---|---|---|---|---|---|
| RefSeq | NM_001099675.1 | NP_001093145.1 | 2 - 2 | 234 | 115 |
| NM_001134646.1 | NP_001128118.1 | 3 - 3 | 284 | 165 | |
| GenBank mRNA | AA423234.1 | - | 2 - 2 | 234 | 115 |
| AK003822.1 | - | 2 - 2 | 230 | 115 | |
| CA466100.1 | - | 3 - 3 | 276 | 165 | |