The assay primers and probes lie within a single exon. This assay will detect gDNA.
| Interrogated Sequence | Translated Protein | Exon Boundary | Assay Location | Amplicon Length | |
|---|---|---|---|---|---|
| RefSeq | NM_001271768.1 | NP_001258697.1 | 5 - 5 | 4875 | 77 |
| NM_024469.2 | NP_077789.1 | 5 - 5 | 4746 | 77 | |
| GenBank mRNA | AK032333.1 | - | 1 - 1 | 2556 | 77 |
| Gene Name | Gene Symbol | Alias | Distance | Relative Direction | Location Chromosome | |
|---|---|---|---|---|---|---|
| Upstream | Ras association (RalGDS/AF-6) domain family (N-terminal) member 8 | Rassf8 | 5133400D11Rik, AA815821, AW123240, Hoj1, mHoj-1 | -37.2kb | Anti-sense | Chr. - 145746562 - 145821079 on Build GRCm38 |
| Downstream | sarcospan | Sspn | Krag | 69.1kb | Anti-sense | Chr. - 145931150 - 145965225 on Build GRCm38 |