The amplified product spans an exon junction and the probe and one of the primers sit within one exon. This assay may detect gDNA.
Interrogated Sequence | Translated Protein | Exon Boundary | Assay Location | Amplicon Length | |
---|---|---|---|---|---|
GenBank mRNA | BC031665.1 | - | 2 - 2 | 131 | 105 |
BC098322.1 | - | 2 - 2 | 121 | 105 | |
U59760.1 | - | 2 - 2 | 83 | 105 |