The amplified product spans an exon junction and the probe and one of the primers sit within one exon. This assay may detect gDNA.
| Interrogated Sequence | Translated Protein | Exon Boundary | Assay Location | Amplicon Length | |
|---|---|---|---|---|---|
| RefSeq | NM_001106487.1 | NP_001099957.1 | 5 - 5 | 761 | 63 |
| XM_006234449.1 | XP_006234511.1 | 6 - 6 | 797 | 63 | |
| XM_006234450.1 | XP_006234512.1 | 6 - 6 | 780 | 63 | |