The amplified product spans an exon junction and the probe and one of the primers sit within one exon. This assay may detect gDNA.
Interrogated Sequence | Translated Protein | Exon Boundary | Assay Location | Amplicon Length | |
---|---|---|---|---|---|
RefSeq | NM_053309.1 | NP_445761.1 | 2 - 2 | 399 | 291 |
GenBank mRNA | AB007689.1 | - | 2 - 2 | 399 | 291 |
AB007690.1 | - | 2 - 2 | 213 | 291 |