The amplified product spans an exon junction and the probe and one of the primers sit within one exon. This assay may detect gDNA.
| Interrogated Sequence | Translated Protein | Exon Boundary | Assay Location | Amplicon Length | |
|---|---|---|---|---|---|
| RefSeq | NM_001244150.1 | NP_001231079.1 | 3 - 3 | 372 | 73 |
| XM_005667370.3 | XP_005667427.1 | - | 498 | 73 | |
| GenBank mRNA | AF053629.1 | - | 2 - 2 | 152 | 73 |
| AK231706.1 | - | 3 - 3 | 285 | 73 | |
| AK231867.1 | - | 3 - 3 | 281 | 73 | |
| AK234135.1 | - | 3 - 3 | 336 | 73 | |
| AK236759.1 | - | 3 - 3 | 281 | 73 | |
| AK238084.1 | - | 3 - 3 | 286 | 73 | |
| AK345464.1 | - | 3 - 3 | 256 | 73 | |
| AK393811.1 | - | 3 - 3 | 286 | 73 | |
| AK393849.1 | - | 3 - 3 | 285 | 73 | |
| AK397718.1 | - | 3 - 3 | 411 | 73 | |
| AK397931.1 | - | 3 - 3 | 286 | 73 | |
| AK398562.1 | - | 3 - 3 | 285 | 73 | |
| AK399019.1 | - | 3 - 3 | 308 | 73 | |
| AK400885.1 | - | 3 - 3 | 285 | 73 | |
| AY610393.1 | - | 3 - 3 | 259 | 73 | |