The amplified product spans an exon junction and the probe and one of the primers sit within one exon. This assay may detect gDNA.
Interrogated Sequence | Translated Protein | Exon Boundary | Assay Location | Amplicon Length | |
---|---|---|---|---|---|
RefSeq | NM_001037998.2 | NP_001033087.1 | 4 - 4 | 853 | 59 |
XM_021081941.1 | XP_020937600.1 | - | 865 | 59 | |
XM_021081945.1 | XP_020937604.1 | - | 886 | 59 | |
GenBank mRNA | DQ288285.1 | - | 4 - 4 | 854 | 59 |