The amplified product spans an exon junction and the probe and one of the primers sit within one exon. This assay may detect gDNA.
| Interrogated Sequence | Translated Protein | Exon Boundary | Assay Location | Amplicon Length | |
|---|---|---|---|---|---|
| RefSeq | NM_001097479.1 | NP_001090948.1 | 4 - 4 | 350 | 106 |
| GenBank mRNA | AK343368.1 | - | 4 - 4 | 106 | |
| AK399809.1 | - | 4 - 4 | 385 | 106 | |
| AY610378.1 | - | 3 - 3 | 340 | 106 | |
| DQ629167.1 | - | 4 - 4 | 350 | 106 | |