The amplified product spans an exon junction and the probe and one of the primers sit within one exon. This assay may detect gDNA.
| Interrogated Sequence | Translated Protein | Exon Boundary | Assay Location | Amplicon Length | |
|---|---|---|---|---|---|
| RefSeq | NM_001123125.1 | NP_001116597.1 | 2 - 2 | 445 | 114 |
| XM_005665170.3 | XP_005665227.1 | - | 546 | 114 | |
| XM_005665171.3 | XP_005665228.2 | - | 714 | 114 | |
| GenBank mRNA | DQ917644.1 | - | 2 - 2 | 445 | 114 |