The assay is designed to a sequence that does not exhibit typical exon-intron-exon gene structure, such as a fusion transcript, or an artificial, prokaryotic or mitochondrial sequence.
| Interrogated Sequence | Translated Protein | Exon Boundary | Assay Location | Amplicon Length | |
|---|---|---|---|---|---|
| RefSeq | NM_001005071.1 | NP_001005071.1 | 5 - 6 | 1133 | 81 |
| XM_012960880.2 | XP_012816334.1 | 6 - 7 | 876 | 81 | |
| XM_018093003.1 | XP_017948492.1 | 6 - 7 | 890 | 81 | |
| XM_018093004.1 | XP_017948493.1 | 6 - 7 | 987 | 81 | |
| XM_018093005.1 | XP_017948494.1 | 6 - 7 | 1190 | 81 | |
| XM_018093006.1 | XP_017948495.1 | 6 - 7 | 848 | 81 | |
| GenBank mRNA | BC076980.1 | - | 5 - 6 | 1133 | 81 |