The amplified product spans an exon junction and the probe and one of the primers sit within one exon. This assay may detect gDNA.
| Interrogated Sequence | Translated Protein | Exon Boundary | Assay Location | Amplicon Length | |
|---|---|---|---|---|---|
| RefSeq | NM_174767.2 | NP_777192.1 | 1 - 1 | 147 | 113 |
| GenBank mRNA | AF251032.1 | - | 1 - 1 | 147 | 113 |
| BC133552.1 | - | 1 - 1 | 399 | 113 | |