Assay Design

The assay probe spans an exon junction. This assay will detect gDNA.

Gene Transcripts

Gene Symbol: Eb1
Entrez Gene ID: 35584
Gene Name: +TIP End-Binding 1
Gene Aliases: BcDNA.LD08743, BcDNA:LD08743, CG3265, Dm EB1, DmEB1, Dmel\CG3265, Dmel_CG3265, EB-1, EB1, S(DmcycE[JP])2.5, anon-EST:Liang-2.33, anon-EST:Liang-2.52, clone 2.33, clone 2.52, dEB-1, dEB1, dEb1, dmEB1, eb1, l(2)04524, l(2)4524
Chromosome Location: Chr.2R: 6749227 - 6756300 on Build Release_6_plus_ISO1_MT
Species Specific ID (Flybase ID): FBgn0027066

Interrogated Sequence		Translated Protein	Exon Boundary	Assay Location	Amplicon Length
RefSeq	NM_001273805.1	NP_001260734.1	6 - 7	857	130
	NM_136389.5	NP_610233.1	6 - 7	950	130
	NM_165487.4	NP_724495.2	6 - 7	968	130
	NM_165488.3	NP_724496.1	6 - 7	950	130
	NM_165489.3	NP_724497.1	6 - 7	856	130
	NM_206030.3	NP_995752.1	6 - 7	947	130
GenBank mRNA	AF132560.1	-	6 - 7	940	130
	AY071380.1	-	6 - 7	984	130
	BT100289.1	-	6 - 7	942	130