Assay Design

The assay primers and probes lie within a single exon. This assay will detect gDNA.

Gene Transcripts

Gene Symbol
Pepck1
Entrez Gene ID
37131
Gene Name
Phosphoenolpyruvate carboxykinase 1
Gene Aliases
143299_at, CG10924, CG17725, Dmel\CG17725, Dmel_CG17725, Dromel_CG17725_FBtr0086701_pepck_mORF, PEPCK, Pepck, dPEPCK, pepck, pepck1
Chromosome Location
Chr.2R: 18536767 - 18539416 on Build Release_6_plus_ISO1_MT
Species Specific ID (Flybase ID)
FBgn0003067
Interrogated Sequence Translated Protein Exon Boundary Assay Location Amplicon Length
RefSeq NM_079060.3 NP_523784.2 2 - 2 2384 120
GenBank mRNA BT003447.1 - 2 - 2 2383 120

Gene Symbol
CG45087
Entrez Gene ID
19834964
Gene Name
CG45087-PA
Gene Aliases
Dmel\CG45087, Dmel_CG45087, Dromel_CG17725_FBtr0086701_pepck_uORF
Chromosome Location
Chr.2R: 18536767 - 18539416 on Build Release_6_plus_ISO1_MT
Species Specific ID (Flybase ID)
FBgn0266488
Interrogated Sequence Translated Protein Exon Boundary Assay Location Amplicon Length
RefSeq NM_001299647.1 NP_001286576.1 2 - 2 2384 120

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