Assay Design

The assay primers and probes lie within a single exon. This assay will detect gDNA.

Gene Transcripts

Gene Symbol: MFGE8
Entrez Gene ID: 4240
Gene Name: milk fat globule-EGF factor 8 protein
Gene Aliases: BA46, EDIL1, HMFG, HsT19888, MFG-E8, MFGM, OAcGD3S, SED1, SPAG10, hP47
Chromosome Location: Chr.15: 88898683 - 88913469 on Build GRCh38

Interrogated Sequence		Translated Protein	Exon Boundary	Assay Location	Amplicon Length
RefSeq	XM_017022206.1	XP_016877695.1	6 - 6	2288	57
GenBank mRNA	AK092157.1	-	1 - 1	1952	57

Non Gene Transcripts

Interrogated Sequence		Translated Protein	Exon Boundary	Assay Location	IMAGE Clone ID	Transcript Location	Amplicon Length
GenBankmRNA	DB323133.1	-	1			Chr. 15 - 88902186 - 88902728 [+] on Build GRCh38	57
GenBankmRNA	CN482859.1	-	1	402		Chr. 15 - 88902593 - 88903096 [-] on Build GRCh38	57
GenBankmRNA	BG740880.1	-	1	224		Chr. 15 - 88902105 - 88902917 [-] on Build GRCh38	57
GenBankmRNA	CN294740.1	-	1	154		Chr. 15 - 88902114 - 88902847 [-] on Build GRCh38	57

Adjacent Gene Details

	Gene Name	Gene Symbol	Alias	Distance	Relative Direction	Location Chromosome
Upstream	hyaluronan and proteoglycan link protein 3	HAPLN3	EXLD1, HsT19883	-6.6kb	Sense	Chr. - 88877285 - 88895626 on Build GRCh38
Downstream	abhydrolase domain containing 2	ABHD2	HS1-2, LABH2, PHPS1-2	183.5kb	Anti-sense	Chr. - 89088150 - 89202360 on Build GRCh38

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