The amplified product spans an exon junction and the probe and one of the primers sit within one exon. This assay may detect gDNA.
| Interrogated Sequence | Translated Protein | Exon Boundary | Assay Location | Amplicon Length | |
|---|---|---|---|---|---|
| RefSeq | NM_001170755.2 | NP_001164226.1 | 5 - 5 | 553 | 75 |
| GenBank mRNA | AK097180.1 | - | 5 - 5 | 472 | 75 |