The amplified product spans an exon junction and the probe and one of the primers sit within one exon. This assay may detect gDNA.
| Interrogated Sequence | Translated Protein | Exon Boundary | Assay Location | Amplicon Length | |
|---|---|---|---|---|---|
| RefSeq | NM_010272.1 | NP_034402.1 | 1 - 1 | 463 | 144 |
| GenBank mRNA | AF092734.1 | - | 1 - 1 | 208 | 144 |