The amplified product spans an exon junction and the probe and one of the primers sit within one exon. This assay may detect gDNA.
| Interrogated Sequence | Translated Protein | Exon Boundary | Assay Location | Amplicon Length | |
|---|---|---|---|---|---|
| RefSeq | NM_010920.2 | NP_035050.2 | 2 - 2 | 268 | 88 |
| GenBank mRNA | AF030113.1 | - | 2 - 2 | 82 | 92 |
| AF045150.1 | - | 2 - 2 | 268 | 88 | |
| AK006401.1 | - | 2 - 2 | 124 | 92 | |
| AK007304.1 | - | 2 - 2 | 51 | 88 | |
| BC100425.1 | - | 2 - 2 | 86 | 88 | |
| BC132558.1 | - | 2 - 2 | 118 | 88 | |
| BC139035.1 | - | 2 - 2 | 118 | 88 | |
| BC145516.1 | - | 2 - 2 | 122 | 92 | |