Assay Design

The assay primers and probes lie within a single exon. This assay will detect gDNA.

Gene Transcripts

Gene Symbol: Plekhf1
Entrez Gene ID: 72287
Gene Name: pleckstrin homology domain containing, family F (with FYVE domain) member 1
Gene Aliases: 1810013P09Rik, APPD, LAPF, PHAFIN1, ZFYVE15
Chromosome Location: Chr.7: 38220654 - 38227994 on Build GRCm38

Interrogated Sequence		Translated Protein	Exon Boundary	Assay Location	Amplicon Length
RefSeq	NM_024413.2	NP_077724.2	2 - 2	1454	85
GenBank mRNA	AK007489.1	-	1 - 1	862	85
	AK143976.1	-	-	1443	85
	AK157990.1	-	2 - 2	1443	85
	AK170283.1	-	2 - 2	1453	85
	BC002120.1	-	1 - 1	1420	85