The amplified product spans an exon junction and the probe and one of the primers sit within one exon. This assay may detect gDNA.
| Interrogated Sequence | Translated Protein | Exon Boundary | Assay Location | Amplicon Length | |
|---|---|---|---|---|---|
| RefSeq | NM_001100834.1 | NP_001094304.1 | 2 - 2 | 240 | 142 |
| GenBank mRNA | BC099794.1 | - | 2 - 2 | 240 | 142 |
| FQ215196.1 | - | 2 - 2 | 192 | 142 | |
| FQ215675.1 | - | 2 - 2 | 192 | 142 | |